Composite

Part:BBa_K3939998:Design

Designed by: Lingwei Ding   Group: iGEM21_Tianjin   (2021-09-30)


Cas9 with flipped promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 749
    Illegal BglII site found at 1686
    Illegal BamHI site found at 2504
    Illegal XhoI site found at 4042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3238
    Illegal AgeI site found at 402
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to flip the promoter, one of the loxp sequence should be reverse


Source

from lab

References